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SOX2 antibody (AA 89-119)

Name: SOX2 antibody (AA 89-119)
SKU: ABIN3032736
Price: 606.92 USD
Availability: InStock

SOX2 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated

Western blot analysis of SOX-2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (2). (SOX2 antibody (AA 89-119))

Flow cytometric analysis of NCI-H292 cells using SOX-2 antibody (bottom histogram) compared to a negative control cell (top histogram). (SOX2 antibody (AA 89-119))

FACS

IHC analysis of FFPE human lung carcinoma tissue stained with SOX-2 antibody (SOX2 antibody (AA 89-119))

IHC

Western blot analysis of SOX-2 antibody and 293 lysate (SOX2 antibody (AA 89-119))

Immunofluorescence analysis of SOX-2 antibody and HeLa cells. (SOX2 antibody (AA 89-119))

IHC

8 images

Catalog No. ABIN3032736

Datasheet as PDF

Target See all SOX2 Antibodies

SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
Binding Specificity
18

16

16

15

15

15

13

11

10

10

9

8

7

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 89-119
Reactivity
201

95

69

21

13

10

10

10

8

8

7

7

5

5

2

2

1

1

1
Human
Host
164

42

2
Rabbit
Compatible Secondaries
Clonality
163

45
Polyclonal
Conjugate
102

16

15

10

10

9

3

3

3

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1
This SOX2 antibody is un-conjugated
Application
164

109

46

44

43

40

40

28

25

23

17

16

12

6

4

4

4

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Cross-Reactivity (Details)

Expected species reactivity: Mouse, Chicken, Zebrafish, Xenopus

Purification

Purified

Immunogen

A portion of amino acids 89-119 from the human protein was used as the immunogen for this SOX-2 antibody.

Isotype

Ig Fraction

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anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody
KO Validated SOX2 Reactivity: Human WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 227-317) antibody
SOX2 Reactivity: Human, Mouse, Rat, Goat WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 1-140) antibody
SOX2 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 1-317) antibody
SOX2 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 89-119), (N-Term) antibody
SOX2 Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB14026 unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 176-305) antibody
SOX2 Reactivity: Human, Mouse WB, ELISA, IHC, Coat, StM Host: Mouse Monoclonal SOX2-1791 unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (N-Term) antibody
SOX2 Reactivity: Human, Mouse, Rat, Goat, Pig, Cow, Sheep, Zebrafish (Danio rerio), Horse, Rabbit, Dog WB, IHC Host: Rabbit Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody
SOX2 Reactivity: Human WB, ELISA, IHC, ICC Host: Mouse Monoclonal 10F10 unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (Middle Region) antibody
SOX2 Reactivity: Human, Mouse, Rat, Goat, Cow, Sheep, Zebrafish (Danio rerio), Horse, Rabbit, Dog, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) (AA 251-284) antibody
SOX2 Reactivity: Human WB, IF, FACS Host: Rabbit Polyclonal RB14259 unconjugated

Application Notes

Titration of the SOX-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the SOX-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name

SOX-2 (SOX2 Products)

Synonyms

ANOP3 antibody, MCOPS3 antibody, RGD1565646 antibody, Sox-2 antibody, lcc antibody, ysb antibody, cb236 antibody, wu:fb83g04 antibody, wu:fc14d07 antibody, zgc:65860 antibody, zgc:77389 antibody, SRY-box2 antibody, Xsox-2 antibody, anop3 antibody, mcops3 antibody, sox-2 antibody, SRY-box 2 antibody, SRY box 2 antibody, SRY (sex determining region Y)-box 2 antibody, SRY-box 2 S homeolog antibody, SOX2 antibody, Sox2 antibody, sox2 antibody, sox2.S antibody

Background

The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.

UniProt

P48431

Pathways

Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis