SMAD4 antibody (AA 400-428)
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- Target See all SMAD4 Antibodies
- SMAD4 (SMAD Family Member 4 (SMAD4))
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Binding Specificity
- AA 400-428
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SMAD4 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Cross-Reactivity (Details)
- Expected species reactivity: Mouse, Rat, Bovine, Pig
- Purification
- Purified
- Immunogen
- A portion of amino acids 400-428 from the human protein was used as the immunogen for this SMAD4 antibody.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product SMAD4 Primary Antibody
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anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody
SMAD4 Reactivity: Human WB, IP, CUT&RUN Host: Rabbit Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 1-552) antibodySMAD4 Reactivity: Human WB, ELISA, IF, PLA Host: Mouse Monoclonal 3E2 unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodyKO Validated SMAD4 Reactivity: Human WB, IHC, IP Host: Rabbit Monoclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 255-284) antibodySMAD4 Reactivity: Human, Mouse WB, IF Host: Rabbit Polyclonal RB14714 unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 Reactivity: Human WB, ELISA, IHC, ICC, FACS Host: Mouse Monoclonal 4G1C6 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 27-195) antibodySMAD4 Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 400-428), (C-Term) antibodySMAD4 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB20720 unconjugated
anti-SMAD Family Member 4 (SMAD4) (pThr277) antibodySMAD4 Reactivity: Human ELISA, IHC, IF, DB Host: Rabbit Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 9-231) antibodySMAD4 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 Reactivity: Human, Mouse, Rat WB, IHC (p), IF (cc) Host: Rabbit Monoclonal 3A1 unconjugated
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- Application Notes
- Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Target
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Alternative Name
- SMAD4 (SMAD4 Products)
- Background
- In muscle physiology, SMAD4 plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). [UniProt]
- UniProt
- Q13485
- Pathways
- Cell Division Cycle, Chromatin Binding, Autophagy
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