SKP2 antibody (AA 156-185)

Catalog No. ABIN3032602

Product Details anti-SKP2 Antibody

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Binding Specificity: AA 156-185

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purification: Purified

Immunogen: A portion of amino acids 156-185 from the human protein was used as the immunogen for this SKP2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the SKP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SKP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2 (SKP2 Products)

Synonyms: FBXL1 antibody, FBL1 antibody, FLB1 antibody, p45 antibody, RGD1562456 antibody, S-phase kinase-associated protein 2 (p45) antibody, S-phase kinase associated protein 2 antibody, Skp2 antibody, SKP2 antibody

Background: SKP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class, in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas.

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases