Rb Protein
Reactivity: Human, Cow, Dog, Pig, Bat, Guinea Pig
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Titration of the Retinoblastoma antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the Retinoblastoma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
Retinoblastoma Protein (Rb) (Rb Protein)
(Retinoblastoma Protein (Rb Protein))
RB1/Retinoblastoma is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.