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PTEN antibody (AA 69-104)

PTEN Reactivity: Human WB, ELISA, IF, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3032214
  • Target See all PTEN Antibodies
    PTEN (Phosphatase and Tensin Homolog (PTEN))
    Binding Specificity
    • 18
    • 17
    • 16
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 69-104
    Reactivity
    • 46
    • 17
    • 17
    Human
    Host
    • 45
    • 1
    Rabbit
    Clonality
    • 45
    • 1
    Polyclonal
    Conjugate
    • 31
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PTEN antibody is un-conjugated
    Application
    • 25
    • 21
    • 14
    • 14
    • 13
    • 13
    • 5
    • 5
    • 3
    • 2
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
    Purification
    Purified
    Immunogen
    A portion of amino acids 69-104 from the human protein was used as the immunogen for this PTEN antibody.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product PTEN Primary Antibody
  • Application Notes
    Titration of the PTEN antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In 1X PBS pH 7.4 with 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot the PTEN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
  • Target
    PTEN (Phosphatase and Tensin Homolog (PTEN))
    Alternative Name
    PTEN (PTEN Products)
    Background
    PTEN, (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), is a tumor suppressor implicated in a large number of human tumors. The PTEN phosphatase incorporates the catalytic motif (HCXXGXXRS/T) that is a signature of the protein tyrosine phosphatase family. Recombinant human PTEN is a dual phosphatase with ability to dephosphorylate both tyrosine and serine/threonine residues. PTEN functions primarily as a lipid phosphatase to regulate signal transduction pathways, with a primary target identified as phosphatidylinositol 3,4,5 trisphosphate. In addition, PTEN presents weak tyrosine phosphatase activity, which may downregulate signaling pathways involving focal adhesion kinase or Shc. PTEN negatively regulates activation of the serine/threonine kinase Akt/PKB by blocking its phosphorylation, thereby inhibiting the PI 3 kinase Akt signaling pathway, which is important for cell survival. In vivo, the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity. Mutations in PTEN are associated with several common cancers including prostate, brain and breast cancer, and with Cowden's disease, an autosomal dominant disorder conferring susceptibility to benign and malignant tumors. Germline mutations of PTEN are also linked Lhermitte-Duclos disease and Bannayan-Zonana syndrome. Mutations of PTEN occur in 60 to 80 % of prostate cancers. PTEN is also essential for embryonic development.
    UniProt
    P60484
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Inositol Metabolic Process, Synaptic Membrane, Regulation of Cell Size, Autophagy, Platelet-derived growth Factor Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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