PRMT5 antibody (AA 591-621)
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- Target See all PRMT5 Antibodies
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
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Binding Specificity
- AA 591-621
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PRMT5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Cross-Reactivity (Details)
- Expected species reactivity: Mouse, Bovine, Primate
- Purification
- Purified
- Immunogen
- A portion of amino acids 591-621 from the human protein was used as the immunogen for this PRMT5 antibody.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product PRMT5 Primary Antibody
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- Application Notes
- Titration of the PRMT5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot the PRMT5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Target
- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
- Alternative Name
- PRMT5 (PRMT5 Products)
- Background
- Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
- UniProt
- O14744
- Pathways
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
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