PKM2 antibody (AA 116-145)

Catalog No. ABIN3032239

Product Details anti-PKM2 Antibody

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Binding Specificity: AA 116-145

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rabbit, Rat

Purification: Purified

Immunogen: A portion of amino acids 116-145 from the human protein was used as the immunogen for this PKM2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PKM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2 (PKM2 Products)

Background: PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

UniProt: P14618

Pathways: Warburg Effect