PITX2 antibody (AA 122-151)

Catalog No. ABIN3032141

Product Details anti-PITX2 Antibody

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Binding Specificity: AA 122-151

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PITX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Chicken, Zebrafish, Xenopus

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 122-151 from the human protein was used as the immunogen for this PITX2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PITX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PITX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PITX2

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Alternative Name: PITX2 (PITX2 Products)

Background: Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.

UniProt: Q99697

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development