ErbB2/Her2 antibody (pTyr1127)

Catalog No. ABIN3029500

Product Details anti-ErbB2/Her2 Antibody

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Binding Specificity: pTyr1127

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB)

Purification: Antigen affinity purified

Immunogen: This phos-ERBB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1127 of human HER2/ERBB2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phos-ERBB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-5000,Immunofluorescence: 1:100,Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phos-ERBB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: Erbb2 (ErbB2/Her2 Products)

Background: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development