Titration of the PDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the PDK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Sirait-Fischer, Olesch, Fink, Berkefeld, Huard, Schmid, Takeda, Brüne, Weigert: "Immune Checkpoint Blockade Improves Chemotherapy in the PyMT Mammary Carcinoma Mouse Model." in: Frontiers in oncology, Vol. 10, pp. 1771, (2020) (PubMed).
PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.