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PCNA antibody (AA 231-261)

Name: PCNA antibody (AA 231-261)
SKU: ABIN3032321
Price: 649.42 USD
Availability: InStock

PCNA Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of PCNA antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the PCNA gene. (PCNA antibody (AA 231-261))

Confocal immunofluorescent analysis of PCNA antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PCNA antibody (AA 231-261))

Confocal immunofluorescent analysis of PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PCNA antibody (AA 231-261))

IHC analysis of FFPE human hepatocarcinoma tissue stained with PCNA antibody (PCNA antibody (AA 231-261))

IHC

PCNA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma. (PCNA antibody (AA 231-261))

IHC

Western blot analysis of PCNA antibody and Jurkat, HeLa, 293 lysate (PCNA antibody (AA 231-261))

IHC

10 images

Catalog No. ABIN3032321

Datasheet as PDF

Target See all PCNA Antibodies

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
Binding Specificity
16

16

16

15

12

11

8

6

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 231-261
Reactivity
210

150

149

54

28

27

21

19

19

18

17

14

11

10

6

6

5

5

4

4

4

3

3

2

2

2

2

2

1

1

1

1

1
Human
Host
134

89

3
Rabbit
Compatible Secondaries
Clonality
134

91

1
Polyclonal
Conjugate
107

16

12

8

5

5

5

5

5

5

5

5

5

5

5

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
Application
186

68

61

59

50

46

46

40

29

15

15

11

5

4

3

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity (Details)

Expected species reactivity: Rat, Bovine, Primate, Hamster

Purification

Purified

Immunogen

A portion of amino acids 231-261 from the human protein was used as the immunogen for this PCNA antibody.

Isotype

Ig Fraction

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PCNA Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Rhesus Monkey WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1H10 unconjugated

Application Notes

Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name

PCNA (PCNA Products)

Background

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

UniProt

P12004

Pathways

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy