MUSK antibody

Catalog No. ABIN3031880

Product Details anti-MUSK Antibody

Target: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUSK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Purified

Immunogen: This mouse Musk antibody was produced from a mouse immunized with recombinant protein from a mouse Musk.

Clone: 1429CT456-173-44

Isotype: IgG1 kappa

Application Details

Application Notes: Titration of the Musk antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Western blot: 1?2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Musk antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MUSK

Target: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Alternative Name: Musk (MUSK Products)

Synonyms: unp antibody, fj98e12 antibody, zgc:63467 antibody, wu:fj98e12 antibody, si:dz46m7.2 antibody, si:dz57f23.3 antibody, si:busm1-57f23.3 antibody, musk-A antibody, MuSK antibody, MUSK antibody, Mdk4 antibody, Mlk antibody, Nsk1 antibody, Nsk2 antibody, Nsk3 antibody, muscle antibody, muscle associated receptor tyrosine kinase antibody, muscle, skeletal, receptor tyrosine kinase antibody, muscle associated receptor tyrosine kinase S homeolog antibody, MUSK antibody, musk antibody, musk.S antibody, Musk antibody

Background: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.

UniProt: Q61006

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development