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LSD1 antibody (AA 819-852)

KDM1A Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3031653
  • Target See all LSD1 (KDM1A) Antibodies
    LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))
    Binding Specificity
    • 15
    • 7
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 819-852
    Reactivity
    • 74
    • 36
    • 30
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 57
    • 15
    • 1
    Rabbit
    Clonality
    • 57
    • 17
    Polyclonal
    Conjugate
    • 54
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LSD1 antibody is un-conjugated
    Application
    • 66
    • 33
    • 24
    • 20
    • 17
    • 13
    • 13
    • 10
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Cross-Reactivity (Details)
    Expected species reactivity: Mouse
    Purification
    Purified
    Immunogen
    A portion of amino acids 819-852 from the human protein was used as the immunogen for this LSD1 antibody.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product KDM1A Primary Antibody
  • Application Notes
    Titration of the LSD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In 1X PBS, pH 7.4, with 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot the LSD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
  • Target
    LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))
    Alternative Name
    LSD1 (KDM1A Products)
    Background
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. [UniProt]
    UniProt
    O60341
    Pathways
    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect
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