LIN28B antibody (AA 95-128)

Catalog No. ABIN3031627

Product Details anti-LIN28B Antibody

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Binding Specificity: AA 95-128

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN28B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Cross-Reactivity (Details): Expected species reactivity: Mouse

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Alternative Name: LIN28B (LIN28B Products)

Synonyms: csdd2 antibody, LIN-28 antibody, CSDD2 antibody, 2810403D23Rik antibody, D030047M17Rik antibody, Lin-28.2 antibody, lin-28 homolog B antibody, lin-28 homolog B S homeolog antibody, lin-28 homolog B (C. elegans) antibody, LIN28B antibody, lin28b antibody, lin28b.S antibody, Lin28b antibody

Background: LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). [UniProt]

UniProt: Q6ZN17