ISG15 antibody (AA 31-61)

Catalog No. ABIN3031502

Product Details anti-ISG15 Antibody

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Binding Specificity: AA 31-61

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 31-61 from the human protein was used as the immunogen for this ISG15 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the ISG15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:50-1:100,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ISG15 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15 (ISG15 Products)

Synonyms: G1P2 antibody, IFI15 antibody, IP17 antibody, UCRP antibody, hUCRP antibody, ISG15 antibody, g1p2 antibody, si:rp71-1c23.2 antibody, G1p2 antibody, IGI15 antibody, Irfp antibody, ISG17 antibody, ISG17/UCRP antibody, ISG15 ubiquitin-like modifier antibody, ISG15 antibody, isg15 antibody, Isg15 antibody

Background: ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.

UniProt: P05161