IGF1R antibody (AA 51-77)

Catalog No. ABIN3031345

Product Details anti-IGF1R Antibody

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Binding Specificity: AA 51-77

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat

Purification: Purified

Immunogen: A portion of amino acids 51-77 from the human protein was used as the immunogen for this IGF1R antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the IGF1R antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the IGF1R antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R (IGF1R Products)

Background: IGF1R is a tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1 receptor is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis.

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy