HLA-DQA1
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
Biotin
Application Notes
Titration of the HLA-DQA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the HLA-DQA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
HLA-DQA1
(Major Histocompatibility Complex, Class II, DQ alpha 1 (HLA-DQA1))
CD antibody, CELIAC1 antibody, DQ-A1 antibody, GSE antibody, HLA-DQA antibody, BoLA-DQA antibody, hla-dqa1 antibody, major histocompatibility complex, class II, DQ alpha 1 antibody, major histocompatibility complex, class II, DQ alpha, type 1 antibody, major histocompatibility complex, class II, DQ alpha 1 L homeolog antibody, HLA-DQA1 antibody, BOLA-DQA1 antibody, hla-dqa1.L antibody
Background
HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.