Titration of the HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,ChIP: 1:100
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS pH 7.4 with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. NSJBio's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.