HIST1H4A antibody (meLys20)

Catalog No. ABIN3031210

Product Details anti-HIST1H4A Antibody

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Binding Specificity: meLys20

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H4A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB), Flow Cytometry (FACS)

Purification: Antigen affinity purified

Immunogen: Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the H4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for HIST1H4A

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Alternative Name: H4 (HIST1H4A Products)

Synonyms: Hist1h4a antibody, H4FA antibody, histone cluster 1, H3f antibody, histone cluster 1 H4 family member a antibody, histone cluster 1, H4a antibody, Hist1h3f antibody, HIST1H4A antibody, Hist1h4a antibody

Background: Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

UniProt: P62805