GST-Tag antibody
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- Target See all GST-Tag products
- GST-Tag
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Reactivity
- Schistosoma japonicum
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GST-Tag antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purification
- Purified
- Immunogen
- Purified recombinant fusion protein was used to produced this monoclonal GST tag antibody.
- Clone
- 9AT106
- Isotype
- IgG1
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- Application Notes
- Titration of the GST Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 1X PBS pH 7.4 with 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot the GST Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Target
- GST-Tag
- Alternative Name
- GST Tag (GST-Tag Products)
- Target Type
- Tag
- Background
- Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
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