Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
Purification
Antigen affinity purified
Immunogen
This GLI2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1287-1321 amino acids from the C-terminal region of human GLI2.
Verified
GLI2
Reactivity: Human
ELISA, IHC
Host: Goat
Polyclonal
unconjugated
Application Notes
Titration of the GLI2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. GLI-2 exists as five alternatively spliced isoforms of 133 kDa (isoform 1), 132 (2), 88 (3), 86 (4) and 168 kDa (isoform 5).\. Immunofluorescence: 1:25,Flow Cytometry: 1:25,Western blot: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the GLI2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody
Background
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Is involved in the smoothened (SHH) signaling pathway.