GAK antibody (AA 15-45)

Catalog No. ABIN3030600

Product Details anti-GAK Antibody

Target: GAK (Cyclin G Associated Kinase (GAK))

Binding Specificity: AA 15-45

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Expected species reactivity: Rat

Purification: Purified

Immunogen: A portion of amino acids 15-45 from the human protein was used as the immunogen for this GAK antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the GAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:50-1:100,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the GAK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GAK

Target: GAK (Cyclin G Associated Kinase (GAK))

Alternative Name: GAK (GAK Products)

Synonyms: GAK antibody, DKFZp469F1313 antibody, gak antibody, MGC172462 antibody, si:dkeyp-1e8.1 antibody, DNAJ26 antibody, DNAJC26 antibody, D130045N16Rik antibody, cyclin G associated kinase antibody, GAK antibody, gak antibody, Gak antibody

Background: GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain.

UniProt: Q6P490