Actin, gamma 1 antibody (AA 188-215)

Catalog No. ABIN3029799

Product Details anti-Actin, gamma 1 Antibody

Target: Actin, gamma 1 (ACTG1)

Binding Specificity: AA 188-215

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Chicken, Zebrafish, Rabbit, Xenopus, Drosophila, C. elegans

Purification: Purified

Immunogen: A portion of amino acids 188-215 from human ACTG1 was used as the immunogen for this g-Actin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the g-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the g-Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Actin, gamma 1

Target: Actin, gamma 1 (ACTG1)

Alternative Name: Actin (gamma, ACTG1) (ACTG1 Products)

Background: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, is a cytoplasmic actin found in nonmuscle cells.

UniProt: P63261

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization