UBD antibody (AA 120-153)

Catalog No. ABIN3029430

Product Details anti-UBD Antibody

Target: UBD (Ubiquitin D (UBD))

Binding Specificity: AA 120-153

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 120-153 from the human protein was used as the immunogen for this FAT10 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the FAT10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the FAT10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for UBD

Target: UBD (Ubiquitin D (UBD))

Alternative Name: FAT10 (UBD Products)

Synonyms: FAT10 antibody, GABBR1 antibody, UBD-3 antibody, ubiquitin D antibody, UBD antibody, Ubd antibody

Background: FAT10/UBD is a Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). [UniProt]

UniProt: O15205

Pathways: Ubiquitin Proteasome Pathway