EED antibody (AA 269-303)

Catalog No. ABIN3030801

Product Details anti-EED Antibody

Target: EED (Embryonic Ectoderm Development (EED))

Binding Specificity: AA 269-303

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antigen affinity purified

Immunogen: This EED antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 269-303 amino acids from the central region of mouse Eed.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the EED antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the EED antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED (EED Products)

Synonyms: Xeed antibody, heed antibody, wait1 antibody, EED antibody, HEED antibody, WAIT1 antibody, zgc:112509 antibody, Xeed-B antibody, eed antibody, psXEED antibody, ENSMUSG00000039373 antibody, l(7)5Rn antibody, l7Rn5 antibody, lusk antibody, Xeed-A antibody, embryonic ectoderm development antibody, embryonic ectoderm development S homeolog antibody, microRNA 6755 antibody, embryonic ectoderm development L homeolog antibody, eed antibody, EED antibody, eed.S antibody, MIR6755 antibody, Eed antibody, eed.L antibody

Background: Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.

UniProt: Q921E6