CDK5 antibody (AA 254-289)

Catalog No. ABIN3030614

Product Details anti-CDK5 Antibody

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Binding Specificity: AA 254-289

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Cross-Reactivity (Details): Expected species reactivity: Rat, Bovine, Xenopus

Purification: Antigen affinity purified

Immunogen: This CDK5 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 254-289 amino acids from the C-terminal region of human CDK5.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CDK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Flow Cytometry: 1:25,Immunofluorescence: 1:25,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CDK5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5 (CDK5 Products)

Synonyms: PSSALRE antibody, AW048668 antibody, Crk6 antibody, CDK5 antibody, CG8203 antibody, DmCdk5 antibody, Dmel\\CG8203 antibody, cdk5 antibody, zgc:101604 antibody, cyclin dependent kinase 5 antibody, cyclin-dependent kinase 5 antibody, Cyclin-dependent kinase 5 antibody, cyclin-dependent kinase 5 L homeolog antibody, Cyclin-dependent-like kinase 5 antibody, CDK5 antibody, Cdk5 antibody, cdk5 antibody, cdk5.L antibody, cdk-5 antibody

Background: Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity, synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity.[UniProt]

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis