CDK4 antibody (AA 273-305)

Catalog No. ABIN3030613

Product Details anti-CDK4 Antibody

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Binding Specificity: AA 273-305

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Purified

Immunogen: A portion of amino acids 273-305 from the human protein was used as the immunogen for this CDK4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CDK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4 (CDK4 Products)

Synonyms: zgc:153726 antibody, CMM3 antibody, PSK-J3 antibody, Crk3 antibody, 8-6 antibody, CDK4 antibody, CDK4/6 antibody, CG5072 antibody, Cdk4/6 antibody, DmCdk4 antibody, Dmel\\CG5072 antibody, Pk53C antibody, Pk?7 antibody, cdk antibody, cdk4 antibody, cdk4/6 antibody, dCdk4 antibody, l(2)05428 antibody, l(2)0671 antibody, l(2)k06503 antibody, l(2)s4639 antibody, l(2)sh0671 antibody, xcdk4 antibody, cyclin-dependent kinase 4 antibody, cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, cyclin-dependent kinase 4 L homeolog antibody, cdk4 antibody, CDK4 antibody, Cdk4 antibody, cdk4.L antibody

Background: CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size