CDK4 antibody (AA 272-303)

Catalog No. ABIN3030610

Product Details anti-CDK4 Antibody

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Binding Specificity: AA 272-303

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 272-303 from the rat protein was used as the immunogen for this Cdk4 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Immunofluorescence: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Cdk4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cdk4 (CDK4 Products)

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

UniProt: P35426

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size