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CD4 antibody (AA 58-86)

Name: CD4 antibody (AA 58-86)
SKU: ABIN3030390
Price: 606.92 USD
Availability: InStock

CD4 Reactivity: Human FACS, WB, ELISA Host: Rabbit Polyclonal unconjugated

CD4 antibody flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). (CD4 antibody (AA 58-86))

FACS

Western blot analysis of CD4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (2). (CD4 antibody (AA 58-86))

Western blot analysis of CD4 antibody and CEM lysate; Expected size: 40~60 kDa depending on glycosylation level (CD4 antibody (AA 58-86))

FACS

5 images

Catalog No. ABIN3030390

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Binding Specificity
29

16

16

16

16

9

7

6

5

4

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 58-86
Reactivity
329

200

109

46

24

20

19

18

14

10

10

10

10

8

7

6

6

6

6

5

2

2

1

1

1

1
Human
Host
319

143

106

3

3

1
Rabbit
Compatible Secondaries
Clonality
433

137

3
Polyclonal
Conjugate
184

66

58

46

34

14

12

10

10

9

9

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD4 antibody is un-conjugated
Application
453

187

130

89

87

66

55

42

39

34

29

25

10

7

7

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

Purification

Antigen affinity purified

Immunogen

A portion of amino acids 58-86 from the human protein was used as the immunogen for this CD4 antibody.

Isotype

Ig Fraction

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CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Pig, Dog, Cat, Rabbit, Cow, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat IF, IHC (p) Host: Mouse Monoclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

Application Notes

Titration of the CD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50

Restrictions

For Research Use only
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Aliquot the CD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Synonyms

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

UniProt

P01730

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events