ATG12 antibody (AA 1-50)
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- Target See all ATG12 Antibodies
- ATG12 (Autophagy Related 12 (ATG12))
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Binding Specificity
- AA 1-50
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATG12 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Purification
- Antigen affinity purified
- Immunogen
- A portion of amino acids 1-50 from the human protein was used as the immunogen for this ATG12 antibody.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product ATG12 Primary Antibody
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- Application Notes
- Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:50-1:100
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot the ATG12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Target
- ATG12 (Autophagy Related 12 (ATG12))
- Alternative Name
- ATG12 (ATG12 Products)
- Background
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
APG12L/ATG12 is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
- UniProt
- O94817
- Pathways
- Autophagy
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