This Alkaline Phosphatase antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
Purification
Antigen affinity purified
Immunogen
A portion of amino acids 274-304 from human ALPI was used as the immunogen for this anti-Alkaline Phosphatase antibody. This sequence is 87% homologous in human ALPP.
ALP
Reactivity: Human
ELISA, IEM
Host: Sheep
Polyclonal
unconjugated
Application Notes
Titration of the anti-Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-100,Immunofluorescence: 1:100
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the anti-Alkaline Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.