Recombinant Histone 3 antibody (2meLys9)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- 2meLys9
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Reactivity
- All Species
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)
- Characteristics
- This recombinant H3K9me2 antibody reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated (K9me1) or trimethylated Lysine 9 (K9me3), or other methylation in Histone H3.
- Purification
- Protein A purified from animal origin-free supernatant
- Immunogen
- A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9) was used as the immunogen for this recombinant H3K9me2 antibody.
- Clone
- RM151
- Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
- The stated application concentrations are suggested starting points. Titration of the recombinant H3K9me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.25-1 μg/mL,ChIP: 2-10 μg/mg of lysate,Immunocytochemistry: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL
- Restrictions
- For Research Use only
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- Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store the recombinant H3K9me2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Background
- This recombinant H3K9me2 antibody reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated (K9me1) or trimethylated Lysine 9 (K9me3), or other methylation in Histone H3.
- Gene ID
- 8350
- UniProt
- P84243
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