Recombinant 5-Hydroxymethylcytosine antibody

Catalog No. ABIN4953286

The Rabbit Monoclonal anti-5-Hydroxymethylcytosine antibody has been validated for ELISA, DB, hmeDIP, ICC and IHC. It is suitable to detect 5-Hydroxymethylcytosine in samples from .

Quick Overview for Recombinant 5-Hydroxymethylcytosine antibody (ABIN4953286)

Target: 5-Hydroxymethylcytosine (5-hmC)

Antibody Type: Recombinant Antibody

Reactivity: Please inquire

Host: Rabbit

Clonality: Monoclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: ELISA, Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Clone: RM236

Product Details anti-5-Hydroxymethylcytosine Antibody

Characteristics: This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine (5hmC)

Target Type: Chemical

Background: This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.