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Recombinant 5-Hydroxymethylcytosine antibody

5-hmC ELISA, DB, hmeDIP, ICC, IHC Host: Rabbit Monoclonal RM236 unconjugated Recombinant Antibody
Catalog No. ABIN4953286
  • Target See all 5-Hydroxymethylcytosine (5-hmC) products
    5-Hydroxymethylcytosine (5-hmC)
    Antibody Type
    Recombinant Antibody
    Reactivity
    Please inquire
    Host
    • 11
    • 5
    Rabbit
    Clonality
    • 10
    • 6
    Monoclonal
    Conjugate
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    This 5-Hydroxymethylcytosine antibody is un-conjugated
    Application
    • 8
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Characteristics
    This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
    Purification
    Protein A purified from animal origin-free supernatant
    Immunogen
    BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.
    Clone
    RM236
    Isotype
    IgG
  • Application Notes
    The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

    1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL

    Restrictions
    For Research Use only
  • Buffer
    1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
  • Target
    5-Hydroxymethylcytosine (5-hmC)
    Alternative Name
    5-Hydroxymethylcytosine (5hmC) (5-hmC Products)
    Target Type
    Chemical
    Background
    This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
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