CD14 antibody (AA 292-322)

Catalog No. ABIN3030357

Product Details anti-CD14 Antibody

Target: CD14

Binding Specificity: AA 292-322

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Purified

Immunogen: A portion of amino acids 292-322 from the human protein was used as the immunogen for this CD14 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4°C for up to one month. For long term, aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: In concert with LBP, CD14 binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades