Optimal dilution of the CYP1A1 antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Frozen): 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the CYP1A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
CYP1A1
(Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
AHH antibody, AHRR antibody, CP11 antibody, CYP1 antibody, P1-450 antibody, P450-C antibody, P450DX antibody, Cyp1a2 antibody, P450-1 antibody, cyp1a1 antibody, CYP1A1 antibody, CYPIA1 antibody, Cyp45c antibody, Cypc45c antibody, P-450MC antibody, wu:fb63b04 antibody, zfCYP1A antibody, zgc:109747 antibody, ahh antibody, ahrr antibody, cp11 antibody, cyp1 antibody, cyp1a antibody, p1-450 antibody, p450-c antibody, p450dx antibody, cytochrome P450 family 1 subfamily A member 1 antibody, cytochrome P450 1A1 antibody, cytochrome P450, family 1, subfamily a, polypeptide 1 antibody, cytochrome P450, family 1, subfamily A, polypeptide 1 antibody, cytochrome P450 family 1 subfamily D polypeptide 1 antibody, cytochrome P4501A1 antibody, polycyclic hydrocarbon-inducible cytochrome P450c antibody, cytochrome P450, family 1, subfamily A antibody, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 antibody, cytochrome P450 family 1 subfamily A member 1 L homeolog antibody, CYP1A1 antibody, CpipJ_CPIJ010542 antibody, Cyp1a1 antibody, cyp1d1 antibody, LOC100328613 antibody, cyp1a antibody, LOC102129994 antibody, cyp1a1.L antibody
Background
CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo(a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo(a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo(a)pyrene carcinogen into the systemic circulation.