The optimal dilution of the FSP1 antibody for each application should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Preservative
Azide free
Storage
4 °C,-20 °C
Storage Comment
Store the FSP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Target
s100a4 (S100A4)
(S100 Calcium Binding Protein A4 (S100A4))
S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. FSP1 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, alpha6beta4 integrin, and liprin b1. FSP1 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.