Alkaline Phosphatase antibody

Catalog No. ABIN4949437

Product Details anti-Alkaline Phosphatase Antibody

Target: Alkaline Phosphatase (ALP)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.

Clone: ALPL-597

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Alkaline Phosphatase antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase (ALP Products)

Synonyms: ALP antibody, ALP1 antibody, CRASH antibody, ECK0378 antibody, JW0374 antibody, psiA antibody, 2410004D18Rik antibody, AU040643 antibody, AW060375 antibody, ILC antibody, CTACK antibody, ESkine antibody, Akp2 antibody, alp antibody, zgc:56672 antibody, DDBDRAFT_0205491 antibody, DDBDRAFT_0231570 antibody, DDB_0205491 antibody, DDB_0231570 antibody, Actn2lp antibody, Alp antibody, asparaginase like 1 antibody, bacterial alkaline phosphatase antibody, C-C motif chemokine ligand 27 antibody, alkaline phosphatase, liver/bone/kidney antibody, alkaline phosphatase antibody, PDZ and LIM domain 3 antibody, ASRGL1 antibody, phoA antibody, Asrgl1 antibody, CCL27 antibody, alpl antibody, alp antibody, Pdlim3 antibody

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.