PAK4 antibody
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- Target See all PAK4 Antibodies
- PAK4 (P21-Activated Kinase 4 (PAK4))
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Reactivity
- Human, Mouse, Rat, Dog
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PAK4 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Recognizes p21 activated kinase 4 (PAK4).
- Characteristics
- Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4
- Purification
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
- Components
- incl. positive Control
- Immunogen
- Peptide conjugated to hemocyaninEpitope: Peptide derived from the kinase activation loop.
- Clone
- 6C1
- Isotype
- IgG1
- Top Product
- Discover our top product PAK4 Primary Antibody
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- Application Notes
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Positive Control Provided. Cell lysate from untreated SW480 cellsDescription: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
- Expiry Date
- 12 months
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- Target
- PAK4 (P21-Activated Kinase 4 (PAK4))
- Alternative Name
- PAK4 (PAK4 Products)
- Synonyms
- 5730488L07Rik antibody, AW555722 antibody, mKIAA1142 antibody, im:5916170 antibody, wu:fd12b01 antibody, zgc:92014 antibody, p21 (RAC1) activated kinase 4 antibody, p21 protein (Cdc42/Rac)-activated kinase 4 antibody, PAK4 antibody, Pak4 antibody, pak4 antibody
- Background
- P21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
- Gene ID
- 10298
- UniProt
- O96013
- Pathways
- RTK Signaling
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