The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
FAS
Reactivity: Human
WB, ELISA, IP
Host: Mouse
Monoclonal
1B6
unconjugated
Application Notes
Flow cytometry: 10 μL reagent / 100 μL of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light. Shelf life: one year from despatch.
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6