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ZAP70 antibody

ZAP70 Reactivity: Human, Mouse WB, FACS, IF, IHC (p) Host: Mouse Monoclonal 4C2 unconjugated
Catalog No. ABIN4915233
  • Target See all ZAP70 Antibodies
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Reactivity
    • 269
    • 125
    • 48
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    Human, Mouse
    Host
    • 205
    • 60
    • 2
    • 2
    • 1
    Mouse
    Clonality
    • 198
    • 71
    Monoclonal
    Conjugate
    • 133
    • 16
    • 15
    • 12
    • 11
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This ZAP70 antibody is un-conjugated
    Application
    • 192
    • 116
    • 102
    • 65
    • 53
    • 53
    • 43
    • 22
    • 22
    • 22
    • 13
    • 12
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Cross-Reactivity
    Human, Mouse
    Purification
    Purified by Protein G.
    Immunogen
    This Zap70 antibody is generated from a mouse immunized with a recombinant protein.
    Clone
    4C2
    Isotype
    IgG2a
    Top Product
    Discover our top product ZAP70 Primary Antibody
  • Application Notes
    WB 1:300-5000
    FCM 1:20-100
    IHC-P 1:200-400
    IF()
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for 12 months.
    Expiry Date
    12 months
  • Target
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Alternative Name
    Zap70 (ZAP70 Products)
    Synonyms
    SRK antibody, STCD antibody, STD antibody, TZK antibody, ZAP-70 antibody, 70kDa antibody, AI327364 antibody, Srk antibody, mrtle antibody, mur antibody, zap70 antibody, MGC80670 antibody, MGC88962 antibody, ZAP70 antibody, zgc:110383 antibody, zeta chain of T-cell receptor associated protein kinase 70 antibody, putative tyrosine-protein kinase zap-70 antibody, zeta-chain (TCR) associated protein kinase antibody, zeta chain of T cell receptor associated protein kinase 70 antibody, zeta-chain (TCR) associated protein kinase 70kDa antibody, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antibody, zeta chain of T cell receptor associated protein kinase 70kDa antibody, tyrosine-protein kinase ZAP-70 antibody, ZAP70 antibody, Smp_149470 antibody, Zap70 antibody, zap70.L antibody, zap70 antibody, LOC707555 antibody, LOC100342021 antibody, LOC100390922 antibody, LOC100542877 antibody
    Background

    Synonyms: Srk, mur, mrtle, ZAP-70, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, Zap70

    Background: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

    Gene ID
    22637
    UniProt
    P43404
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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