VAMP8 antibody

Catalog No. ABIN4915080

This anti-VAMP8 antibody is a Mouse Monoclonal antibody detecting VAMP8 in WB and IHC (p). Suitable for Human.

Quick Overview for VAMP8 antibody (ABIN4915080)

Target: VAMP8 (Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 14C3

Product Details anti-VAMP8 Antibody

Cross-Reactivity: Human

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This VAMP8 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 2~24 amino acids from the N-terminal region of human VAMP8.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for VAMP8

Target: VAMP8 (Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8))

Alternative Name: VAMP8

Background:

Synonyms: EDB, VAMP-8, Vesicle-associated membrane protein 8, Endobrevin, VAMP8

Background: SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).

Gene ID: 8673

UniProt: Q9BV40