ULK3 antibody

Catalog No. ABIN4915037

Product Details anti-ULK3 Antibody

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ULK3 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This ULK3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 435-468 amino acids from the C-terminal region of human ULK3.

Clone: 4C5

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ULK3

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Alternative Name: ULK3 (ULK3 Products)

Synonyms: zgc:162196 antibody, 1200015E14Rik antibody, unc-51 like kinase 3 antibody, unc-51 like kinase 3 L homeolog antibody, unc-51-like kinase 3 antibody, ULK3 antibody, ulk3 antibody, Ulk3 antibody, ulk3.L antibody

Background:

Synonyms: Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3

Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Gene ID: 25989

UniProt: Q6PHR2