HCK antibody

Catalog No. ABIN4910490

Product Details anti-HCK Antibody

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: This HCK antibody is generated from a mouse immunized with a recombinant protein of human HCK.

Clone: 8C1

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: HCK (HCK Products)

Synonyms: laloo antibody, HCK antibody, MGC107870 antibody, JTK9 antibody, p59Hck antibody, p61Hck antibody, AI849071 antibody, Bmk antibody, Hctk antibody, p56Hck antibody, HCK proto-oncogene, Src family tyrosine kinase L homeolog antibody, HCK proto-oncogene, Src family tyrosine kinase antibody, hemopoietic cell kinase antibody, hck.L antibody, HCK antibody, hck antibody, Hck antibody

Background:

Synonyms: JTK9, p59Hck, p61Hck, Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, HCK

Background: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Gene ID: 3055

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling