NLRP3 antibody (AA 81-93)

Catalog No. ABIN490652

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: AA 81-93

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: ELISA

Specificity: Human NLRP3. This antibody is expected to recognize all reported isoforms (NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Panda, Bat, Pig (92%) Dog, Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide RDLYEKAKRDEPK-C from the N-terminus (near) of human NLRP3 (NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Panda, Bat, Pig (92%), Dog, Bovine, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 36  kDa band in Human Peripheral Blood Mononucleocytes lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106  kDa according to NP_899632.1. The 36  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NALP3 / NLRP3 (NLRP3 Products)

Background: Name/Gene ID: NLRP3

Synonyms: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS

Gene ID: 114548

NCBI Accession: NP_004886, NP_899632, NP_001120933, NP_001120934

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome