RAD51 antibody

Catalog No. ABIN4902204

Product Details anti-RAD51 Antibody

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rad51 is a protein encoded by the RAD51 gene which is approximately 35 kDa. Rad51 is localised to the nucleus, cytoplasm and mitochondrion matirx. It is involved in the resolution of D-loop structures through synthesis-dependent strand annealing, DNA double-strand break repair, ATM pathway and the BRCA1 pathway. It is known to be involved in the homologous strand exchange, a key step in DNA repair through homologous recombination and repair of DNA. It catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Rad51 is highly expressed in the testis and thymus and less expressed in the small intestine, placenta, colon, pancreas and ovary. Mutations in the RAD51 gene result in breast cancer and Fanconi anemia, a disorder affecting all bone marrow elements. STJ99103 was developed from clone 4H10-G5-H4. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and detects endogenous levels of Rad51 and does not cross-react with related proteins.

Specificity: This antibody detects endogenous levels of Rad51 and does not cross-react with related proteins.

Characteristics: Mouse Monoclonal to Rad51.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant peptide derived from human Rad51.

Clone: 4H10-G5-H4

Isotype: IgG2a

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: Rad51 (RAD51 Products)

Synonyms: BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, MRMV2 antibody, RAD51A antibody, RECA antibody, CRAD51 antibody, wu:fb38e12 antibody, zgc:77754 antibody, RGD1563603 antibody, ATRAD51 antibody, F22D1.20 antibody, F22D1_20 antibody, RAS associated with diabetes protein 51 antibody, AV304093 antibody, Rad51a antibody, Reca antibody, brcc5 antibody, rad51 antibody, rad51a antibody, reca antibody, xrad51 antibody, RAD51 recombinase antibody, RAS associated with diabetes protein 51 antibody, RAD51 recombinase L homeolog antibody, RAD51 antibody, rad51 antibody, Rad51 antibody, rad51.L antibody

Molecular Weight: 37kDa

Gene ID: 5888

UniProt: Q06609

Pathways: DNA Damage Repair