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Luciferase antibody

Reactivity: Firefly WB, IHC (p) Host: Mouse Monoclonal 6B8 unconjugated

Catalog No. ABIN4901900

Datasheet as PDF

Target See all Luciferase Antibodies

Luciferase
Reactivity
26

23

8

7

4

1

1

1
Firefly
Host
50

13

7

1
Mouse
Compatible Secondaries
Clonality
43

28
Monoclonal
Conjugate
31

11

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This Luciferase antibody is un-conjugated
Application
38

24

13

13

13

12

10

8

7

7

5

4

4

3

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose

Luciferase or Luciferin 4-monooxygenase is a protein encoded by the luc gene which is produced in Photinus pyralis or the common eastern firefly.. Luciferase is localised to the peroxisome and is involved in chaperone binding. This protein falls under the ATP-dependent AMP-binding enzyme family and is one of the most extensively studied of the enzymes that catalyse light production in bioluminescent organisms and requires ATP and 02 as substrates. There are two reactions catalysed by luciferase, the first being the formation of an enzyme-bound luciferyl-adenylate and a second in which the luciferyl-adenylate undergoes an oxidative decarboxylation which results in the production of CO2, oxyluciferin, AMP, and green light with a wavelength of 562 nm. STJ97752 was developed from clone 6B8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of Luciferase.

Specificity

Luciferase Mouse Monoclonal Antibody (6B8) detects endogenous levels of Luciferase

Characteristics

Mouse Monoclonal to Luciferase.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen

Recombinant peptide derived from Luciferase

Clone

6B8

Isotype

IgG1

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Reactivity: Photinus pyralis WB Host: Goat Polyclonal unconjugated

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Reactivity: Photinus pyralis WB, IHC Host: Mouse Monoclonal Luci17 unconjugated

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Reactivity: Vibrio fischeri WB, ELISA, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Luciferase antibody
Reactivity: Photinus pyralis WB, ELISA Host: Goat Polyclonal unconjugated

anti-Luciferase antibody
Reactivity: Photinus pyralis WB, IP Host: Mouse Monoclonal 2D4 unconjugated

Application Notes

WB 1:1000-2000
IHC 1:100-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.

Storage

-20 °C

Storage Comment

Store at -20°C, and avoid repeat freeze-thaw cycles.
Target

Luciferase

Abstract

Luciferase Products

UniProt

P08659