H2AFZ antibody (C-Term)

Catalog No. ABIN4889649

Product Details anti-H2AFZ Antibody

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFZ antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Dot Blot (DB), Cleavage Under Targets and Tagmentation (CUT&Tag), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Purification: None

Immunogen: This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-H2AFZ antibody (ABIN4889649)

Law, Cheung: "Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression." in: The Journal of biological chemistry, Vol. 290, Issue 21, pp. 13234-49, (2015) (PubMed).

Gemma, Ramagopalan, Down, Beyan, Hawa, Holland, Hurd, Giovannoni, Leslie, Ebers, Rakyan: "Inactive or moderately active human promoters are enriched for inter-individual epialleles." in: Genome biology, Vol. 14, Issue 5, pp. R43, (2014) (PubMed).

Menet, Pescatore, Rosbash: "CLOCK:BMAL1 is a pioneer-like transcription factor." in: Genes & development, Vol. 28, Issue 1, pp. 8-13, (2014) (PubMed).

Boyarchuk, Filipescu, Vassias, Cantaloube, Almouzni: "The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle." in: Journal of cell science, Vol. 127, Issue Pt 15, pp. 3347-59, (2014) (PubMed).

Lau, Cheung: "Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity." in: Nucleic acids research, Vol. 41, Issue 3, pp. e49, (2013) (PubMed).

Vierstra, Wang, John, Sandstrom, Stamatoyannopoulos: "Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH." in: Nature methods, Vol. 11, Issue 1, pp. 66-72, (2013) (PubMed).

Yu, Feng, Ruan, Komers, Kiviat, Bomsztyk: "Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays." in: BMC molecular biology, Vol. 12, pp. 49, (2011) (PubMed).

El Gazzar, Liu, Yoza, McCall: "Dynamic and selective nucleosome repositioning during endotoxin tolerance." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1259-71, (2010) (PubMed).

Conerly, Teves, Diolaiti, Ulrich, Eisenman, Henikoff: "Changes in H2A.Z occupancy and DNA methylation during B-cell lymphomagenesis." in: Genome research, Vol. 20, Issue 10, pp. 1383-90, (2010) (PubMed).

Target Details for H2AFZ

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Alternative Name: Histone H2A.Z (H2AFZ Products)

Synonyms: H2A.Z-1 antibody, H2A.z antibody, H2A/z antibody, H2AZ antibody, H2A.Z antibody, H2a.z-1 antibody, H2AFV antibody, H2Az antibody, XH2AZ antibody, h2a.zl1 antibody, h2a.zl2 antibody, H2A histone family member Z antibody, H2A histone family, member Z antibody, H2A histone family member Z L homeolog antibody, histone H2A.Z antibody, H2AFZ antibody, H2afz antibody, h2afz.L antibody

Molecular Weight: 14 kDa

Gene ID: 3015

Pathways: Telomere Maintenance