Acetylated Lysine antibody
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- Target See all Acetylated Lysine products
- Acetylated Lysine
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Acetylated Lysine antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- higher plants
- Purification
- affinity purified
- Immunogen
- acetylated KLH
- Clone
- 7F8
- Isotype
- IgG1
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- Application Notes
- 1: 100 (IHC), 1: 1000 (WB), for other assays optimal dilution has to be determined by end user
- Comment
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1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 %glycerol at concentration 1 mg/mL
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. - Storage
- -20 °C
- Storage Comment
- store at -20°C, avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- Acetylated Lysine
- Abstract
- Acetylated Lysine Products
- Synonyms
- C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, KRAS proto-oncogene, GTPase antibody, KRAS antibody
- Target Type
- Chemical
- Background
- Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
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