ZWINT antibody (AA 29-249)

Catalog No. ABIN4886776

Product Details anti-ZWINT Antibody

Target: ZWINT (ZW10 Interactor (ZWINT))

Binding Specificity: AA 29-249

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZWINT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for ZW10 interactor(ZWINT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for ZW10 interactor(ZWINT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: ZW10 interacting kinetochore protein
Protein Name: ZW10 interactor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ZWINT recombinant protein (Position: Q29-Q249). Human ZWINT shares 58% and 57.1% amino acid (aa) sequence identity with mouse and rat ZWINT, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ZWINT

Target: ZWINT (ZW10 Interactor (ZWINT))

Alternative Name: ZWINT (ZWINT Products)

Synonyms: ZWINT antibody, HZwint-1 antibody, KNTC2AP antibody, ZWINT1 antibody, 2010007E07Rik antibody, 2600001N01Rik antibody, D10Ertd749e antibody, Zwint-1 antibody, Sip30 antibody, ZW10 interacting kinetochore protein antibody, ZW10 interactor antibody, ZWINT antibody, Zwint antibody

Background: ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms: HZwint 1 | HZwint1 | KNTC 2 AP | KNTC2AP | ZWINT 1 | ZWINT | Zwint-1 | ZWINT1 | O95229

Gene ID: 11130

UniProt: O95229